hsa-mir-650: Hsa-mir-650 is a microRNA that plays a role in promoting the inflammation-induced apoptosis of intestinal epithelioid cells (IECs) by targeting NLRP6 [PMC8602894]. The primer sequences for PCR analysis of hsa-mir-650 are as follows: miR-650 forward, 5′-AGAGGAGGCAGCGCTCT-3′ and reverse, 5′-CAGTGCGTGTCGTGGAGT-3′ [PMC7509765]. The mature sequence of hsa-mir-650 is 5′-AGGAGGCAGCGCUCUCAGGAC-3′ [PMC7509765]. Hsa-mir-650 is a microRNA that has been implicated in the regulation of various cellular processes. In the context provided, it has been shown to promote inflammation-induced apoptosis of intestinal epithelioid cells (IECs) by targeting NLRP6, a protein involved in the regulation of inflammation and cell death [PMC8602894]. This suggests that hsa-mir-650 may play a role in modulating the inflammatory response and cell survival in the intestine. To study hsa-mir-650, PCR analysis was performed using specific primer sequences. The forward primer sequence for miR-650 was 5′ - AGAGGAGGCAGCGCTCT - 3′ and the reverse primer sequence was 5′ - CAGTGCGTGTCGTGG AGT - 3' [PMC7509765]. These primers were designed to specifically amplify hsa-mir-650 during PCR analysis. The mature sequence of hsa-mir-650 was determined to be 5' - AGG AGGC AGC GCUCUC AGGAC - 3' [PMC7509765]. This mature sequence represents the functional form of hsa-mir-650 and is important for its regulatory activity. In conclusion, hsa-mir-650 is a microRNA that promotes inflammation-induced apoptosis of intestinal epithelioid cells by targeting NLRP6. PCR analysis using specific primer sequences and the determination of the mature sequence of hsa-mir-650 provide important tools for studying its function and regulatory mechanisms [PMC8602894] [PMC7509765].