LINC00314: LINC00314 is a long non-coding RNA (lncRNA) that plays a critical role in the regulation of osteogenic differentiation of adipose-derived stem cells (ADSCs) [PMC7302136]. It is located on chromosome 21 with open reading frame 94 [PMC7302136]. LINC00314 was found to be significantly upregulated during the osteogenic differentiation of ADSCs [PMC7302136]. It acts as a sponge for miR-129-5p, thereby promoting the expression of GRM5, a gene involved in the Wnt/β-catenin signaling pathway [PMC7302136]. Overexpression of LINC00314 was shown to enhance osteogenic differentiation, as indicated by increased expression of osteogenic markers and increased ALP activity and calcified nodule formation [PMC7302136]. On the other hand, silencing LINC00314 suppressed these effects [PMC7302136]. The interaction between LINC00314 and miR-129-5p was predicted using bioinformatics analysis and confirmed by experimental data [PMC7302136]. The binding site between LINC00314 and miR-129-5p was identified using bioinformatics databases [PMC7302136]. The role and mechanism of LINC00314 in ADSC osteogenic differentiation had not been previously explored before this study [PMC7302136]. The study also identified other lncRNAs that may play a role in osteoporosis development or management, including MALAT1, NEAT1, DANCR, SNHG1, MIR22HG, and LINC00314 [PMC10137190]. In conclusion, LINC00314 is an upregulated lncRNA during ADSC osteogenic differentiation that acts as a sponge for miR-129-5p to promote GRM5 expression through the Wnt/β-catenin signaling pathway, thereby enhancing osteogenic differentiation [PMC7302136].