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dme-mir-7: Dme-mir-7 is a specific miRNA assay control for Drosophila melanogaster (D. melanogaster) [PMC6224079]. It was used in a pilot run to confirm the absence of plant/insect miRNA contamination and unspecific amplifications in serum samples [PMC6224079]. Dme-mir-7 was also included in a Taqman Low-Density Array (TLDA) card for qPCR analysis of mature human miRNAs [PMC6224079]. In a study comparing different miRNAs, dme-mir-7 was found to have 5325 recovered regions, with the SBM method returning 28 [PMC2365947]. The dme-mir-7 results showed an average of 273 sequences scoring equal to or better than the left-out sequences and an average of 8868 sequences scoring at least as high as the validated target using miRanda [PMC2365947]. In another study, dme-mir-7 was used as a spike-in RNA for RNA isolation and reverse transcription in serum samples [PMC8615628]. It was also found to be significantly down-regulated in SSc-A-MSCs compared to HC-A-MSCs, with a fold change less than 0.5 [PMC6509164]. The cDNA synthesized from total RNA using dme-mir-7 RT-primer was used for TaqMan MicroRNA Assay analysis [PMC4273950].
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