osa-miR444a-3p.2: osa-mir444a-3p.2 is a miRNA that has been implicated in Al stress responses [PMC6069884]. In a study comparing two different varieties of barley, XZ29 and Golden Promise, osa-mir444a-3p.2 was found to be down-regulated in XZ29 and showed little change in Golden Promise [PMC6069884]. The target gene of osa-mir444a-3p.2, HORVU2Hr1G08490.1 (MADS27), was up-regulated in both XZ29 and Golden Promise [PMC6069884]. In another study using a weighted gene co-expression network analysis (WGCNA), osa-mir444a-3p.2 was identified as one of the miRNAs targeted by potential drought-resistant hub genes (MADS47, CCX1, carC, PAO2, and HOX24) [PMC9433978]. This analysis also revealed that osa-mir444a-3p.2 was differentially expressed in A. mongolicum under drought stress and predicted to regulate MADS47 target genes [PMC9433978]. Furthermore, osa-mir444a-3p.2 was found to be one of the miRNAs targeting members of the OsHDZIP subfamily III [PMC9405480]. Additionally, it was observed that osa-mir444a-3p.2 was up-regulated by 4–6 fold under certain conditions [PMC6042804]. Overall, these findings suggest that osa-mir444a-3p.2 may play a role in Al stress responses and drought resistance through its regulation of target genes such as MADS27 and members of the OsHDZIP subfamily III.