cfa-let-7c: Cfa-let-7c is a microRNA that has been predicted to target the tuberous sclerosis 1 (TSC1) gene, which encodes hamartin [PMC4490541]. Target prediction analysis by TargetScan indicated that TSC1 is a predicted gene target for cfa-let-7c [PMC4490541]. In a study comparing different stages of disease in dogs, cfa-let-7c was found to be significantly different between Stages B1/B2 and C/D [PMC4490541]. In the same study, cfa-let-7c was shown to have increased expression in Stage B1/B2 or C/D compared to Stage A [PMC4490541]. Another study found that cfa-miR-26a, cfa-miR-200c, cfa-let-7c, cfa-let-7b, and cfa-let 7a were the top five meaningful miRNAs in relation to aging in dogs [PMC10135127]. MiRNA families such as miR200 and Mirlet 7 were significantly up-regulated with testicular RA intervention [PMC4049822]. In metastatic and non-metastatic mammary tumors, ten miRNAs including cfa let 7c were validated and showed significant different expression [PMC7646326]. In dogs with MMVD and cardiac enlargement or congestive heart failure, four miRNAs including cfa let 7c were upregulated while seven others were downregulated compared to normal dogs [PMC4964495]. The expression of six of these miRNAs also significantly differed between dogs with congestive heart failure and those with cardiac enlargement [PMC4964495]. The ten microRNAs selected for validation of microarray results included cfa let 7c[ PMC5678797] . Serum analysis has shown the downregulation of cfa-miR-302d, cfa-miR-380, cfa-miR-874, cfa-miR-582, cfa-miR-490, cfa-miR-329b and cfa-miR-487b and the upregulation of cfa-miR-103, cfa-miR-98, cfa let 7b and 7c [PMC5533140].