gga-mir-146a: Gga-mir-146a is a differentially expressed miRNA that has been associated with virus infection in both broiler and layer chickens [PMC3496578]. It is one of the miRNAs that are up-regulated after avian influenza virus infection [PMC5389138]. Gga-mir-146a has been found to be involved in the regulation of 3T3-L1 differentiation and adipocyte differentiation [PMC6826404]. It has also been shown to target ESRP2 genes, potentially influencing the postnatal liver maturation process [PMC5758705]. In addition, gga-mir-146a is one of the miRNAs that are up-regulated in CD40L-stimulated B cells, indicating its role in the immune system [PMC3743212]. The expression of gga-mir-146a can be validated using a dual luciferase reporter assay and qPCR [PMC3496578] [PMC8633681]. However, it is not predicted to target chicken IL-2 [PMC2873332]. Overall, gga-mir-146a is a differentially expressed miRNA with potential roles in virus infection, adipocyte differentiation, liver maturation process, and immune system regulation.