bta-mir-532: Bta-mir-532 is a functional miRNA that is abundant in three analyzed tissues and is conserved in humans [PMC6691986]. It has a low miRDeep2 score and lacks a significant randfold p value [PMC6691986]. Bta-mir-532, along with bta-miR-103 and bta-miR-155, shows high homology to human miRNAs [PMC9569736]. Bta-mir-532 is significantly more abundant in corn silage farms compared to grass silage farms [PMC9569736]. It also differs significantly between grazing and zero-grazing farms, with higher abundance in grazing farms [PMC9569736]. Bta-mir-532, along with bta-miR-155, bta-miR-103, and bta-miR-7863, shows significant differences between different dairy production systems [PMC9569736]. These miRNAs may play a role in cell differentiation in the mammary gland and regulate milk production [PMC9569736]. Bta-mir-532 has not been previously studied for its expression in milk [PMC9569736]. It has been associated with spermatogenesis along with other miRNAs such as bta-miR-204 and the bta-miR-34 family [PMC8647812] [PMC6083711] . A false precursor of bta-mir-532 predicted by miRDeep2 contains a short star miRNA, indicating a potential high false positive rate for short precursor-generated miRNAs[ PMC4029070].