cfa-miR-16: Cfa-mir-16 is a microRNA that was used as a reference gene in a study [PMC9235276]. It was chosen as an endogenous reference gene due to its relatively stable expression [PMC7356535]. In the study, an amplification protocol was followed, consisting of specific temperature and time intervals [PMC9235276]. The expression levels of various miRNAs were evaluated, including cfa-miR-122, cfa-miR-885, cfa-miR-1, cfa-miR-133a/b, cfa-miR-208b, cfa-miR-499, cfa-miR-206, cfa-miR-212, cfa-miR-432, cfa-miR-34b/c, cfa-miR-216a/b, and others [PMC4989286]. Three miRNAs (cfa-mir-16,-29a,-186) were selected as normalization controls in the study due to their ubiquitous expression across all tissues [PMC4989286]. The precision of these three highly expressed ubiquitous miRNAs was measured and found to be good in all tissues examined [PMC4989286]. Another study enriched a panel of selected miRNAs with the inclusion of cmiRNA panel comprising of various miRNAs including cmiRNA 320 due to its stability in serum [PMC9951723]. In another analysis of canine miRNA expression patterns in CLL (chronic lymphocytic leukemia), the relative expression pattern of 12 canine miRNAs including mir16 was analyzed [PMC3619122]. Additionally,certain miRNAs including mir16 were selected for qRT PCR verification in another study[ PMC7689213]