osa-miR1846d-5p: Osa-mir1846d-5p is one of the miRNAs that have no predicted target genes, along with osa-miR166j-5p [PMC8551726]. Several heat-responsive miRNAs, including osa-mir1846d-5p, have been identified to target heat shock factors (HSFs) and heat shock proteins (HSPs) to regulate gene expression [PMC9136941]. In the P+H+ condition, the expression of osa-mir1846d-5p is higher compared to P-H+ condition [PMC8067039]. Under priming conditions (P+H-), the expression patterns of osa-mir1846d-5p and other miRNAs such as osa-miR5149, osa-miR5077, osa-miR168a-5p, and osa-miR167e are inversely correlated with the expression of their respective targets [PMC8067039]. These targets include key HSFs and HSPs that are regulated by miRNAs such as osa-mir1846d-5p [PMC8067039]. Osa-mir1846d-5p and other identified miRNAs play a specific role in altering gene expression in response to heat priming [PMC8067039]. The changes in levels of osa-mir1846d-5p and other miRNAs under priming conditions are inversely correlated with changes in their respective transcripts [PMC8067039]. In summary, Osa-mir1846d-5p is a specific type of miRNA that has no predicted target genes. It is involved in regulating gene expression by targeting HSFs and HSPs under heat-responsive conditions. The expression patterns of Osa-mir1846d-5p and other miRNAs are inversely correlated with the expression of their respective targets, and their levels change in response to heat priming. [PMC8551726] [PMC9136941] [PMC8067039].