mmu-mir-185: Mmu-mir-185 is a type of microRNA that has been shown to play a role in regulating osteogenic differentiation of human jaw bone marrow mesenchymal stem cells [PMC9075730]. It has been found that miR-145 can decrease osteogenic differentiation of these cells by targeting semaphorin 3A [PMC9075730]. In addition to miR-145, other microRNAs such as miR-21, miR-148b-3p, mmu-mir-185, and miR-381 have also been found to have important roles in regulating osteogenic differentiation of human mesenchymal stem cells [PMC9075730]. In fact, the CRISPR-Cas9 deletion of mmu-mir-185 has been shown to promote osteogenic differentiation of primary bone-marrow mesenchymal cells [PMC7404082]. The expression level of mmu-mir-185 can be increased by using an mmu-mir-185 expression vector [PMC3708730]. This vector was found to increase the levels of miR-185 approximately fivefold compared to the control [PMC3708730]. Furthermore, the overexpression of miR-185 using this vector was found to affect the functional regulation of mCry1 3'UTR [PMC3708730]. The expression level of mmu-mir-185 has also been quantitated in mouse brain, liver, and heart using the TaqMan MicroRNA assay [PMC1635289]. It was found that mmu-mir 185 is weakly but ubiquitously expressed in these tissues [PMC1635289].