ssc-mir-450a: Ssc-mir-450a is a microRNA that has been found to exhibit consistent up-regulation upon ZEN treatment in both the uterus and serum [PMC6598998]. It is one of several microRNAs that are up-regulated in both the uterus and serum after ZEN treatment, including ssc-miR-1, ssc-miR-542-3p, ssc-miR-143-5p, and others [PMC6598998]. Ssc-mir-450a is also differentially expressed in both the CK and TK groups [PMC9275911]. To validate the sequencing results, qRT-PCR was performed on five randomly selected differentially expressed miRNAs, including ssc-mir-450a [PMC5389138]. In a study on piglets exposed to contaminated feed, ssc-mir-450a was found to be one of several microRNAs commonly affected by ZEN exposure [PMC9142966]. It has also been shown that ZEN affects uterine expression of ssc-mir-450a in pigs [PMC6722729]. In a panel of microRNAs affected by ZEN exposure, ssc-mir-450a was found to be up-regulated along with other miRNAs such as ssc-miR-1 and ssc-miR503 [PMC6722729]. Overall, these findings highlight the role of ssc-mir-450a in response to ZEN exposure and its potential as a biomarker for evaluating the effects of mycotoxins on pig health.