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bmo-mir-8: bmo-mir-8 is a literature-based and database-collected microRNA (miRNA) that has been studied in the context of Bombyx mori (silkworm) and its viral infection. It has been found that miRNAs collected from miRBase, including bmo-mir-8, showed stronger signals compared to other miRNAs [PMC4045974]. Bmo-mir-8 is a host miRNA that targets the mRNA of the viral gene ie-1, a master regulator of the viral infection cycle [PMC5469917]. Blocking bmo-mir-8 resulted in a significant increase in the virus load in infected silkworm larvae [PMC8373394]. The suppression of bmo-mir-8 led to a 3-fold increase in ie-1 transcript level and an 8-fold increase in BmNPV accumulation in fat body tissues of infected larvae [PMC8137832]. Bmo-mir-8 is one of the highly abundant conserved miRNAs found in B. mori [PMC3699532]. It has been identified as an anti-viral miRNA that is suppressed by BmNPV following infection or transfection of abmnpv-miR-1 into host cells [PMC5091789]. Bmo-mir-8, along with other miRNAs like bmo-miR-9a and bmo-miR263a, showed slight elevation after diapause-broken stage (DBS) and maintained relatively constant levels thereafter [PMC2500172]. It is strongly expressed throughout all developmental stages (larva, pupa, and moth) along with other miRNAs like bmo-miR1a and bmolet7a [PMC2435238]. The relative abundance of bmo-mir1a, bmo-mir-8, and bmo-mir-133 in BmN cells has been analyzed using quantitative RT-PCR [PMC4222307]. Bmo-mir-8 has been found to target the immediate-early gene, which can be controlled by bmnpv-miR-1 and Ran dsRNA, resulting in increased virus infection levels in B. mori larvae [PMC4222307].
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