cfa-let-7b: Cfa-let-7b is a microRNA that is part of a NGS-selected cmiRNA panel and has been found to be stable in serum [PMC9951723]. It has been shown to be upregulated in dogs with cardiac hypertrophy and specifically associated with concentric cardiac hypertrophy [PMC8542680]. However, cfa-let-7b alone may be less specific for concentric cardiac hypertrophy compared to cfa-miR-375 [PMC8542680]. The direct target genes and pathways of cfa-let-7b in dogs have not been identified yet [PMC8542680]. In dogs with mitral valve disease (MMVD), cfa-let-7b has been found to be upregulated, along with other microRNAs such as cfa-miR-103, cfa-miR-98, and cfa-miR-375 [PMC4964495]. Cfa-miR-130b has also been found to be upregulated in dogs with MMVD and concentric cardiac hypertrophy [PMC8062772]. In aging dogs, the expression of cfa-miR26a, cfa-miR200c, cfa-let7c, and cfa let7a have been shown to be regulated via PTEN and may play a role in aging processes [PMC10135127]. In the context of different stages of heart disease in dogs, the expression levels of various microRNAs including cfa-miR302d, cfa-miR380, and let7c have been found to differ between stages B1/B2 or C/D compared to stage A or healthy controls [PMC9607079]. The predicted targets for let7b include genes involved in heart disease pathways such as angiotensinogen [PMC4490541].