dme-mir-124: Dme-mir-124 is a microRNA that has been selected based on data generated in the laboratory and its orthology with human miRNAs [PMC5090246]. It has been found to play a role in male sexual differentiation in Drosophila melanogaster by targeting a sex-specific splicing factor in the sex determination pathway [PMC8966896]. Mutant male flies with dme-mir-124 exhibit aberrant pheromone levels, leading to reduced mating success and receptivity by females, as well as increased male-male courtship [PMC8966896]. Dme-mir-124 is similar to vertebrate miR-124a and shows a similar expression pattern to dme-miR-263a and -263b, with trough levels during mid-day followed by increases during the early to late-night in wildtype flies and constantly elevated levels in the cyc0 mutant [PMC2263044]. Among the 78 miRNAs probed for expression profiling, dme-miR263a and -263b displayed strong evidence of circadian regulation, while dme-mir-124 showed possible weak cycling [PMC2263044]. The low apparent sensitivity of sensors for dme-miR-1000, hsa-miR-223–3p, dme-miR-14, and dme-mir-124 was attributed to high background fluorescence in the absence of these miRNAs [PMC4889923].