osa-miR166a-3p: Osa-mir166a-3p is a microRNA that has been studied in various contexts. It is one of the highly expressed miRNAs, with more than 10,000 reads in five different lines [PMC5578646]. It has also been detected along with other known miRNAs, such as osa-mir166a-5p and osa-mir166b-5p [PMC6337123]. Osa-mir166a-3p has been found to be involved in low-light-mediated signaling mechanisms, as shown through expression analysis and validation using qRT-PCR [PMC9614602]. Its expression pattern has been observed to be upregulated in tolerant genotypes and downregulated in sensitive genotypes of rice [PMC9614602]. Osa-mir166a-3p has a negative correlation with the expression of its target gene rolled leaf1, indicating its role in suppressing the expression of rolled leaf1 through cleavage [PMC9614602]. In other studies, osa-mir166a-3p was found to be downregulated in O. alta and O. latifolia, while upregulated in O. officinalis [PMC7662745]. It is also one of the most abundantly expressed conserved miRNAs in DXWR [PMC9960954]. Furthermore, osa-mir166a-3p targets members of the OsHDZIP subfamily III along with other miRNA families [PMC9405480]. qRT-PCR analysis confirmed similar expression patterns for osa-miR156a-5p, osa-mir166a-3p, and osamiR167-a5-p as observed through sequencing data [PMC4203131].