mmu-mir-202: Mmu-mir-202 is a microRNA that is found within the second exon of the lncRNA AK144366, which is down-regulated in neonatal testis [PMC3794988]. This suggests that AK144366 may be the primary precursor to mmu-mir-202 [PMC3794988]. Mmu-mir-202 expression is up-regulated in neonatal testis and down-regulated in adult testis, indicating its potential role in testicular development [PMC3794988]. Mmu-mir-202 has been shown to be one of the small subset of microRNAs found in mitochondria, suggesting its association with mitochondrial function [PMC2905396]. Real-time TaqMan® MicroRNA Assays were used to confirm the expression of mmu-mir-202 and 18 other microRNAs from high-throughput sequencing data [PMC5569061]. In a study on liver tissues, qRT-PCR was used to validate the expression levels of mmu-mir-202 and other miRNAs [PMC4926493]. The reaction conditions for mmu-mir-202 were 15 min at 95 °C, followed by 40 cycles at 95 °C for 20 s, with an annealing temperature of 55 °C for 20 s and an extension at 72 °C for 30 s [PMC5575838]. Overall, these findings highlight the potential role of mmu-mir-202 in testicular development and its association with mitochondrial function.