cfa-mir-222: Cfa-mir-222 is a miRNA that has been identified as a breast cancer-related miRNA in several studies [PMC9917093]. In a study comparing BCMT-T and BCMT-N, cfa-mir-222 was found to be differentially expressed [PMC9917093]. Similarly, in another study comparing MCMT-T and MCMT-N, cfa-mir-222 was also identified as a breast cancer-related miRNA [PMC9917093]. This study was the first to record the significant upregulation of cfa-mir-222 in MCMT [PMC9917093]. However, there have been limited studies conducted on cfa-mir-222 compared to humans [PMC9917093]. To validate the expression levels of miRNAs obtained through high-sequencing, including cfa-mir-222, qPCR was performed on four randomly selected differentially expressed miRNAs [PMC4768678]. In this validation study, cfa-miR-124 was found to be significantly upregulated while there was no significant change observed for cfa-mir-222 in CFDP1_ vs_CFDP2 [PMC4768678]. In summary, cfa-mir-222 is a breast cancer-related miRNA that has been identified as differentially expressed in studies comparing BCMT-T and BCMT-N as well as MCMT-T and MCMT-N. However, there have been limited studies conducted on this particular miRNA compared to humans. Additionally, qPCR validation showed significant upregulation of another miRNA (cfa-miR-124) but no significant change for cfa-mir-222.