rno-mir-222: Rno-mir-222 is a highly abundant miRNA in DHT-treated ovaries [PMC3682887]. It is also exclusively expressed in the theca of cystic follicles [PMC3682887]. Rno-mir-222 has been associated with cardiac hypertrophy [PMC5856749]. It has been found to target Acadm and Echs2 genes [PMC6377737]. MiR-222 knockout rats have been generated using CRISPR technology to study the effects of rno-mir-222 deletion [PMC8626841]. Rno-mir-222, along with rno-miR-221, rno-miR-25, and rno-miR-26b, has been found to be differentially expressed in rats with polycystic ovary syndrome (PCOS) compared to control rats [PMC4838149]. In summary, rno-mir-222 is a highly abundant miRNA in DHT-treated ovaries and is exclusively expressed in the theca of cystic follicles. It has been associated with cardiac hypertrophy and has been found to target Acadm and Echs2 genes. MiR-222 knockout rats have been generated using CRISPR technology. Additionally, rno-mir-222, along with other miRNAs such as rno-miR-221, rno-miR25, and rno-miR26b, is differentially expressed in rats with PCOS compared to control rats.