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bta-mir-99b: Bta-mir-99b is a microRNA that has been studied in various contexts. It has been found to be differentially expressed between fresh and frozen sperm [PMC7214931]. Bta-mir-99b has also been used as a reference gene for normalizing CT data generated by amplification [PMC9831338]. In the study of extracellular vesicles (EVs) isolated from ovarian follicular fluid (OF) and uterine fluid (UF), the expression of bta-mir-99b was used as an internal control for normalizing raw cycle threshold (Ct) values [PMC9594899]. Bta-mir-99b has been found to have a range of predicted targets, from 37 to 803 [PMC7959717]. In the analysis of intestinal regions, bta-mir-99b was found to have a negative correlation with a small number of predicted target genes [PMC7959717]. Bta-mir-99b has also been used as a reference gene for normalizing Ct values in various studies [PMC6525926, PMC5209821, PMC7969882, PMC4517789, PMC6637201]. It has been found to be differentially expressed in milk and plasma during early pregnancy in cattle [PMC6418173]. In response to TLR3 ligand challenge, bta-mir-99b was down-regulated in PBMCs [PMC4892552]. Additionally, higher expression of bta-mir-99b has been observed in tissue exposed to S. aureus compared to unexposed tissue [PMC7937231]. Bta-mir-99b is one of the highly expressed miRNAs in follicular stages during the estrous cycle [PMC4156418]. It is also highly abundant with Ct numbers between 10–25 Ct [PMC5472319]. Furthermore, bta-mir-99b has been found to display significant contrasts between time points in humans [PMC9825267]. It is one of the miRNAs enriched in extracellular vesicles [PMC8036276].
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