dme-mir-274: Dme-mir-274 is a microRNA (miRNA) that plays a central role during embryogenesis/development, as overexpression of dme-mir-274, along with dme-mir-2b and dme-mir-184, resulted in almost total lethality [PMC9100521]. The specific functions of dme-mir-2b and dme-mir-274 in Drosophila are not yet clear [PMC9100521]. However, microarray experiments have shown that both dme-mir-2b and dme-mir-274 are expressed at higher levels in long days compared to short days [PMC9100521]. In fact, seven miRNAs, including dme-mir-2b and dme-mir-274, were found to be differentially expressed between long and short photoperiods [PMC9100521]. These differentially expressed miRNAs (DEMs) were identified through experiments that detected seven DEMs at significant levels (p < 0.01), including dme-mir-2b, dme-mir-11, dme-mir-34, dme-mir-274, dme-miR184*, and 285 [PMC9100521]. To investigate the functional roles of these DEMs in the photoperiodic response of Drosophila diapause (a period of suspended development), overexpression experiments were conducted. Overexpression of specific miRNAs such as DME-MIR 2b, DME-MIR 184*, and DME-MIR 274 using UAS transgenes driven by Act-Gal4 or pdf-Gal4 resulted in changes in photoperiodic diapause [PMC9100521].