hsa-mir-643: Hsa-mir-643 is a microRNA that is predicted to be unique for the polymorphic T allele [PMC2945965]. It was the only microRNA identified using both MicroInspector and PITA [PMC2945965]. Hsa-mir-643, along with hsa-miR-337-3p and hsa-miR-325, was first identified as unique for the polymorphic allele, and RNAhybrid predicted a target on both the polymorphic and wild-type alleles [PMC2945965]. After re-evaluation of RNAhybrid's predictions based on thermodynamic properties, hsa-mir-643 was considered to be the best predicted candidate [PMC2945965]. Future plans include in vitro studies to evaluate the interaction between the polymorphic 3'UTR of the TTR gene and several predicted miRNAs, including hsa-mir-643 [PMC2945965]. Hsa-mir-643 is one of 14 potential hub miRNAs identified in a miRNA network associated with poor prognosis in breast cancer. These hub miRNAs include hsa-mir-320b-2, hsa-mir-545, hsa-mir-941-2, hsa-mir-651, hsa-mir383, hsa-miR1914, hsa-miR570, hsammiR647,hsmmiR1227,hsmmiR593,hsmmiR300,hsmmiR626,andhsmmi R1289.1. The target genes of these hub miRNAs are implicated in cell cycle regulation [PMC8004706]. HSA-MIR 643 is one of several differentially expressed miRNAs identified in hepatocellular carcinoma (LIHC) patients with low and high tumor mutation burden (TMB) [PMC7355149]. HSA-MIR-643 is one of six selected miRNAs whose differential expression was verified using microRNA assays [PMC6333105]. Finally, HSA-MIR-643 is one of two SNP-MPRMs identified for the gene CTTNBP2NL [PMC3279374].