osa-miR169i-5p.2: Osa-mir169i-5p.2 is a member of the miR169 family and is significantly downregulated in both 9311 and DC90 under chilling stress. However, its downregulation level in 9311 is lower than in DC90 [PMC9002458]. Under chilling stress, the expression levels of osa-miR5340, osa-mir169i-5p.2, and osa-miR159a.2 are downregulated, while their predicted target genes (LOC_Os03g15780, LOC_Os05g40720, LOC_Os04g51160, and LOC_Os05g01440) are consistently upregulated [PMC9002458]. Other differentially expressed miRNAs (DEMs) also show significant expression changes under chilling stress. For example, novel_miR_233 and novel_miR_390 are significantly upregulated while osa-mir169i-5p.2 is significantly downregulated in both genotypes [PMC9002458]. In a recent study focusing on root development in rice, osa-mir169i-5p.2 is found to have high abundance in root tips [PMC5447883]. Additionally, a large number of sRNAs including miRNA sequences such as osa-mir169i-5p.2 show high abundances in root tips and whole roots [PMC3734165]. Overall, these findings suggest that osa-mir169i-5p.2 plays an important role under chilling stress and in root development of rice [PMC9002458][PMC5447883][PMC3734165].