gga-mir-23b: Gga-mir-23b is a microRNA that has been reported to be associated with tumorigenesis and the aberrant expression of the retrovirus, ALV-J [PMC5276853]. The bulge-loop RT primer and qPCR primers specific for gga-mir-23b were designed and synthesized [PMC4434839]. Gga-mir-23b has been found to target interferon regulatory factor 1 (IRF1), a critical regulatory protein of the inflammatory response that functions as a tumor suppressor and is involved in cell cycle progression and apoptosis [PMC7063076]. Overexpression of gga-mir-23b resulted in increased expression of gp85, while overexpression of IRF1 caused a decrease in gp85 level [PMC7063076]. Gga-mir-23b promotes the replication of Avian Leukosis virus by repressing the expression of IRF1 [PMC6627052]. It has also been found to be more expressed in the spleen of ALV-J-infected chickens compared to controls, suggesting its important role in ALV-J replication [PMC6862082]. Additionally, gga-mir-23b was found to be expressed at high levels in IAH30 cells along with other miRNAs such as gga-miR-24, gga-miR-27b, gga-miR-19b, gga-miR-20a, gga-miR-148a, and gga-miR92 [PMC3743212]. The enrichment of targets for other miRNAs including gga-miR9*, ggammiR217, ggami-R19a was also significant [PMC5657028].