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ssc-miR-150: ssc-mir-150 is a microRNA that has been identified as a potential target of ssc-miR-185, ssc-F3-C29, ssc-miR-101, ssc-miR-152, and ssc-mir-150 in castrated male pigs [PMC3901342]. It has been found to be differentially regulated in the adipose tissue miRNA of lean pig breeds [PMC7901978]. ssc-mir-150 is believed to directly affect the 3′UTR of CYP3A4 and promote adipose tissue degeneration induced by free fatty acids [PMC7901978]. It has also been predicted to bind to the 3′UTR of WUH3 PRRSV genome along with other microRNAs [PMC5381705]. Additionally, it has been found to be a coupled miRNA with ssc-miR-361-3p and both have several target genes in common, suggesting potential co-regulation [PMC7222767]. The expression levels of ssc-mir-150 have been validated through RNA-seq analysis in different networks [PMC7222767]. The number of differentially expressed isomiRs belonging to ssc-mir-150 varies from one to 17 for other microRNAs [PMC7809845]. qRT-PCR analysis has also confirmed the accuracy of miRNA sequencing data for several microRNAs including ssc-mir-150 [PMC9778086]. In a study comparing high-feed efficiency (FE) and low-FE groups, it was found that the expression levels of both ssc-miR-155-5p and ssc-mir-150 were higher in the high-FE group while the expression levels of other microRNAs including ssc-miR1225p were lower in the high-FE group compared to the low-FE group [PMC9778086].
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