mmu-mir-3470a: Mmu-mir-3470a is a miRNA that was synthesized by the GeneCopoeia Company in China [PMC5148024]. It was selected along with other miRNAs for validation in a study on the correlation between miRNA levels and Comt mRNA expression [PMC5148024]. The study found that there was no in vivo correlation between the levels of mmu-mir-3470a and Comt mRNA, but this does not necessarily mean that mmu-mir-3470a is not functional [PMC4579042]. To confirm its role in pain responses, mmu-mir-3470a was injected into the foot of mice using lentiviral vectors [PMC4579042]. In vitro experiments showed that mmu-mir-3470a can downregulate both the mRNA and enzymatic activity of murine ancestral Comt [PMC4579042]. In vivo experiments using formalin injections demonstrated that mmu-mir-3470a increased immediate licking behavior, indicating increased pain sensitivity [PMC4579042]. The relative expression of mmu-mir-3470a was lower compared to other miRNAs, such as mmu-miR-667-3p [PMC4579042]. Lentiviral injections of mmu-mir-3470a, along with other miRNAs, increased hypersensitivity during inflammatory pain states and downregulated Comt mRNA levels [PMC4579042]. Mmu-miR-667-3p and other selected miRNAs were found to be reliable candidates for regulating full-length Comt transcripts due to their high target accessibility and conservation [PMC4579042]. The relative abundance of all four miRNAs, including mmu-miR6673p, was estimated in different mouse tissues to investigate their association with Comt mRNA levels [PMC4579042].
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