mmu-mir-101a: mmu-mir-101a is a specific miRNA that is differentially expressed during implantation in the mouse uterus and is coordinated with the expression of cyclooxygenase-2 (Cox-2), a gene critical for implantation [PMC3462109]. It has been found that mmu-mir-101a and mmu-miR-199a* are spatiotemporally expressed in the mouse uterus during implantation, along with the expression of Cox-2 [PMC3692437]. These two uterine miRNAs, mmu-mir-101a and mmu-miR-199a*, have been shown to posttranscriptionally regulate a gene critical for implantation [PMC5496128]. In a study on LPS-activated RAW264.7 macrophages, it was analyzed that miR-101 affects the protein level of MKP-1 when transfected with dsRNA mimic or ssRNA inhibitor for mmu-mir-101a [PMC4011752]. The dsRNA mimic for mmu-mir-101a has a sense strand of 5′-UAC AGU ACU GUG AUA ACU GAA -3′ and an antisense strand of 5′ -CAG UUA UCA CAG UAC UGU AUU -3′ [PMC4011752]. The ssRNA inhibitor against mmu-mir-101a has a strand of 5′ -UUC AGU UAU CAC AGU ACU GUA -3′ [PMC4011752]. In mice, it was found that mmu-mir-101a and mmu-miR3653p were downregulated based on miRNA expression profile analysis using microarray analysis [PMC8914318]. The functional significance of uterine expression of mmu-mir 101-a and mmu-miR-199a and their regulation of PTGS2 in a murine embryo implantation model has been demonstrated [PMC3168490]. mmu-mir-101a and mmu-miR-199a have been shown to post-transcriptionally regulate the mRNA expression of cyclooxygenase-2, which is critical for embryonic implantation [PMC5364990]. Additionally, mmu-mir-101a has been found to target the transforming growth factor-beta gene (TGF-beta) and its receptor gene [PMC9198802]. A probe containing a hybridization solution for mmu-mir-101a was added at a concentration of 8 ng/μL during hybridization at 37 °C overnight [PMC7557009].