gga-mir-223: Gga-mir-223 is a microRNA that has been associated with regulating myoblast proliferation in chickens [PMC7936154]. In a study analyzing gga-mir-223-mediated IGF2 signaling in MYOD1-silenced cells, it was found that silencing MYOD1 significantly decreased gga-mir-223 expression and upregulated IGF2 expression [PMC7669912]. The expression of gga-mir-223 and ENSGALG00000004681 was significantly increased after MDV infection in CD4+ T cells, suggesting that the linc-GALMD3 might activate the expression of its downstream neighboring genes through its cis-regulatory effects [PMC5579197]. In MDV-transformed cell lines, gga-miR-155, gga-miR-150, gga-miR-451, gga-miR-26a, and gga-mir-223 were down-regulated compared to normal splenocytes [PMC3511444]. The downregulation of gga-miR-142-3p and gga-mir-223 was observed in IAH30 cells compared to HD11 cells [PMC3743212]. A miRCURY LNA probe for detecting the expression of gga-mir-223 was synthesized [PMC5682648]. In summary, studies have shown that the microRNA gga-mir-223 is involved in regulating myoblast proliferation in chickens. Silencing MYOD1 leads to decreased expression of this microRNA and upregulation of IGF2. Additionally, after MDV infection, the expression of both gga-mir-223 and ENSGALG00000004681 is increased. In MDV-transformed cell lines, several host-encoded miRNAs including gaa-gmiR155 and ga-gmiR26a are downregulated, while gga-mir-223 is upregulated. Furthermore, gga-mir-223 is downregulated in IAH30 cells compared to HD11 cells. A miRCURY LNA probe has been developed to detect the expression of gga-mir-223.