chi-mir-135a: Chi-mir-135a is a miRNA that binds to XR_311113.2 via putative binding sites, along with three other miRNAs (chi-miR-424-5p, chi-miR-544-5p, and chi-miR-3955-5p) [PMC8762113]. Mutants of chi-mir-135a, chi-miR-424-5p, and chi-miR-544-5p were transfected into GK cells to confirm their binding to XR_311113.2 [PMC8762113]. The miRNA binding site regions and mutated regions of chi-mir-135a were cloned into pmirGLO vectors for further analysis [PMC8762113]. XR_311113.2 contains binding sites for all four miRNAs (chi-mir-135a, chi-miR-424-5p, chi-miR544-p, and chi-miR3955-p) [PMC8762113]. Chi-mir135a is also down-regulated in three prenatal stages and the neonatal stage [PMC5087842]. Additionally, there are 17 down-regulated miRNAs that are common between these stages including Chi mir 135a [PMC5087842]. These findings suggest that Chi mir 135a may play a role in prenatal development and neonatal stage regulation along with other down-regulated miRNAs [PMC5087842].