eca-mir-223: Eca-mir-223 is a microRNA that has been found to be expressed at higher levels in old diseased mares compared to young diseased mares and healthy control mares [PMC8227551]. It is one of several microRNAs, including eca-miR-155, eca-miR-200a, and eca-miR-205, that have been shown to target inflammatory immune response genes [PMC8227551]. This study is the first to investigate the expression profile of eca-mir-223 in mares with endometritis [PMC8227551]. The study aimed to measure the expression levels of eca-mir-223 and other microRNAs, as well as the concentrations of IL-6, PGF2α, and PGE2 in serum samples from young and old mares with healthy and abnormal uterine status [PMC8227551]. The results showed higher expression levels of eca-mir-223 in old diseased mares compared to young diseased mares and control mares [PMC8227551]. The over-expression of eca-mir-223 was observed in both young and old diseased mares compared to control healthy mares [PMC8227551]. Eca-mir-223 was found only in microvesicles (MVs) according to RNA-seq data, while other microRNAs such as eca-miR-26 and eca-miR-146a were present in both MVs and cells [PMC6434479]. Real-time PCR validated the expression of eca-miR26, ecam-MIR146a, and ECA-MIR223 in MVs compared to cells [PMC6434479]. These findings suggest that ECA-MIR223 could be a potential target for further research on endometritis.