gga-mir-203: gga-mir-203 is a microRNA that has been found to be significantly differently expressed in all three groups in a study [PMC5203650]. Its expression levels showed big changes in one group (CM) but small changes in another group (CS) [PMC5203650]. The study used qPCR to quantify the expression levels of gga-mir-203 and other miRNAs [PMC4123083]. The researchers also investigated the target relationship between gga-mir-203 and TP63 mRNA using a dual-luciferase reporter gene assay and found that gga-mir-203 can directly bind to the predicted target site of TP63 mRNA [PMC6157316]. TargetScan software predicted that TP63 mRNA is a direct target of gga-mir-203 and the predicted target site is highly conserved among vertebrates [PMC6157316]. The researchers also transfected a gga-mir-203 mimic into chicken primary myoblasts and found that it significantly downregulated the expression of TP63, suggesting that TP63 is a target gene of gga-mir-203 involved in myogenic differentiation [PMC6157316]. Another study identified gga-mir-203 as one of the miRNAs with significant differential abundance in two comparisons, suggesting its potential role in chicken skeletal muscle differentiation [PMC4256448]. Overall, these findings highlight the importance of gga-mir-203 in regulating gene expression and its potential role in myogenic differentiation.

gga-mir-203a: Gga-mir-203a is a microRNA that has been studied in relation to its role in viral replication and immune responses. The target genes of gga-mir-203a, such as TGM2, have been found to be involved in extracellular regions, vesicles, and exosomes, suggesting a potential role in miRNA exocytosis and membrane fusion-mediated interferon production [PMC5983721]. Additionally, gga-mir-203a has been found to be inversely correlated with STAT3 and IL-1R1 expression [PMC5983721]. In the context of NDV replication, gga-mir-203a has been shown to accelerate embryonic death and NDV replication [PMC5983721]. The expression of TGM2 is regulated by gga-mir-203a and plays a negative role in NDV infection [PMC5983721]. The overexpression of gga-mir-203a enhances NDV replication by suppressing TGM2 and deactivating the NF-κB signaling pathway [PMC5983721]. The relationship between gga-mir-203a and TGM2 has been validated through RT-qPCR and luciferase assays [PMC5983721]. In chicken embryos, NDV infection down-regulates the expression of gga-mir-203a, while its overexpression increases NDV replication both in vivo and in vitro by targeting TGM2 [PMC6718473]. Gga-mir-203a is also part of a complex interaction network with other miRNAs such as gga-miR-144-3p and genes involved in viral functions like TGFB2 [PMC5788541][PMC7086581].