LHX5-AS1: LHX5-AS1 is a previously uncharacterized gene cluster that has been identified in a subset of individuals in a cohort study [PMC8012207]. Experimental validation of repeat lengths in LHX5-AS1 using PCR amplification and Sanger sequencing confirmed the predictions made by ExpansionHunter [PMC7844047]. The analysis of the cohort revealed 29 populations characterized by specific genes, including LHX5-AS1, which was found to be strongly enriched at early timepoints and expressed broadly at CS13 but restricted to the developing cortical plate by CS14 and CS16 [PMC8012207]. The expression pattern of LHX5-AS1 RNA was found to be consistent with that of LHX5 protein [PMC8012207]. Additionally, CpG sites related to long noncoding RNA or antisense genes, including LHX5-AS1, were identified and found to be associated with various cell developmental and proliferation pathways in different types of cancer [PMC5687331]. These findings suggest that LHX5-AS1 may play a role in early development and potentially have implications in cancer development. Further research is needed to fully understand the function and significance of LHX5-AS1.