SNORD103A: SNORD103A is a small nucleolar RNA (snoRNA) that was analyzed in a study using qPCR and RNA sequencing techniques [PMC9803687]. The expression of SNORD103A was measured using RT-qPCR and was found to correlate significantly with the small RNA-seq measurements [PMC9803687]. The reverse transcription (RT) step in the analysis of SNORD103A was performed using the TaqMan MicroRNA Reverse Transcription Kit and specific RT primers [PMC9803687]. RT-qPCR was also used to confirm the presence of SNORD103A in a subset of breast tissue samples [PMC9803687]. In addition to SNORD103A, other snoRNAs, such as SNORA80E, SNORD59A, and SNORD104, were also analyzed in the study [PMC9803687]. The study identified various types of snoRNAs, including C/D box snoRNAs (SNORDs) and H/ACA box snoRNAs (SNORA), as well as small nuclear RNAs involved in RNA splicing [PMC7072903]. The distribution of aligned read pairs for SNORD103A and its identical counterpart, SNORD103A, were analyzed using CoCo software [PMC6901076]. It was found that a large proportion of read pairs aligned equally well to both snoRNAs and were discarded as multimapped reads [PMC6901076].