PISRT1: PISRT1 is a non-protein coding gene that is affected by a 11.7 kb deletion in goats with polled/intersex syndrome (PIS) [PMC3494662]. This deletion disrupts the transcription of PISRT1 and two other genes, FOXL2 and PFOXic [PMC2323435]. PISRT1 functions as an antitestis gene by inhibiting SOX9, while FOXL2 promotes ovarian development [PMC2323435]. In goats with XX sex-reversed gonads, the expression of PISRT1 decreases while SOX9 expression increases [PMC2323435]. PISRT1 encodes a 1.5-kb mRNA devoid of an open reading frame (ORF) and is highly expressed in adult testis [PMC2689649]. The deletion that affects PISRT1 also affects the transcription of FOXL2, which is responsible for blepharophimosis epicanthus inversus syndrome in humans [PMC2689649]. The expression levels of PISRT1 were normalized using three housekeeping genes (HPRT1, GAPDH, and YWHAZ) [PMC2689649]. The deletion disrupts a long non-coding RNA (lncRNA) called PISRT1 and eight conserved non-coding sequences (CNCs) [PMC2689649]. In humans, the full-length transcript of PISRT1 was characterized using 5' RACE and its expression was analyzed in various cell lines/tissues using real-time quantitative RT-PCR [PMC2689649]. The deletion that encompasses PISRT1 removes potential regulatory elements necessary for the correct transcription of FOXL2 in goats with polled/intersex syndrome [PMC3597517].