MIR548D1: MIR548D1 is a non-coding RNA gene that produces two mature microRNAs, miR-548d-5p and miR-548d-3p, which target JunD [PMC6959298]. The MIR548D1 promoter luciferase reporter plasmid was constructed by cloning a 1000 bp fragment before the transcription starting site into the pEZX-FR01 plasmid [PMC6959298]. The study aimed to investigate the involvement of FOXD3 and BRD4 in the transcription of MIR548D1, and a luciferase reporter containing its promoter region was generated [PMC6959298]. The study found that FOXD3 is a putative transcription factor for MIR548D1 gene, as predicted by several transcription regulation databases [PMC6959298]. Additionally, bioinformatics analysis of The Cancer Genome Atlas (TCGA) revealed that MIR548D1 is uniquely upregulated in the basal-like subtype of breast cancer compared to other subtypes [PMC6959298]. Furthermore, both mature products of MIR548D1 have putative binding sites on the 3′UTR region of JUND [PMC6959298]. The study also showed that BRD4/FOXD3 was enriched in the promoter region of MIR548D1 through luciferase and sequential ChIP assays [PMC6959298]. Interestingly, FOXD3 expression is also highly expressed in basal-like breast cancer based on TCGA analysis, similar to MIR548D1 expression [PMC6959298]. Finally, among the top 20 upregulated differentially expressed genes (DEGs), eight genes were identified as newly androgen receptor (AR)-regulated genes including CYP4F8 and MIR548D1 [PMC9107748].