MIR5090: MIR5090 is a nitrogen-responsive microRNA (miRNA) candidate in Arabidopsis that plays a role in nitrogen metabolism and adaptation to nitrogen starvation [PMC8539900]. It is involved in the regulation of glucosinolate synthesis, a process important for nitrogen homeostasis [PMC5258760]. MIR5090, along with other miRNAs such as miR826, has been studied for its relationship with nitrogen metabolism in Arabidopsis [PMC9551039]. It targets the gene ALKENYL HYDROXALKYL PRODUCING 2 (AOP2), which is involved in glucosinolate biosynthesis and is repressed by MIR5090 and miR826 under nitrogen starvation conditions [PMC7696010]. MIR5090 and miR826 may have evolved through inverted duplication of the genomic region containing AOP2, suggesting coevolution in response to nitrogen deprivation [PMC7696010]. AOP2 is confirmed as the common target of both MIR5090 and miR826 [PMC4468412]. Overexpression of MIR5090 or miR826 leads to enhanced tolerance to nitrogen limitation and improved growth under nitrogen-deficient conditions [PMC7758431] [PMC9538721]. These findings highlight the role of MIR5090 as a key regulator of nitrogen metabolism and adaptation to nutrient limitation in Arabidopsis.