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SNORD68: SNORD68 is a small nucleolar RNA (snoRNA) that is associated with pre-40S complexes [PMC4288182]. RNAi against the 40S biogenesis factor PWP2 only resulted in a minor reduction in pre-40S associated SNORD68 and SNORD56, while depletion of DDX21 led to a significant reduction of SNORD68 and SNORD56 in pre-40S complexes [PMC4288182]. Etoposide treatment was found to increase the levels of SNORD68 and snord111B, which are the guide RNAs for A2388 and G3923 2′-O-methylation, respectively [PMC6451326]. In stem cell and differentiated cells, normalization was performed using the arithmetic mean of three small RNAs (SNORD68, SNORD95, and SNORD96A) that showed very little or no change [PMC7494815]. To estimate miRNA abundance, a primer targeting both mouse and human mature-miR-451a was used along with an SNORD68 primer targeting both mouse and human SNORD68 [PMC9429931].
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