SNORD116-13: SNORD116-13 is a small nucleolar RNA (snoRNA) that is part of the SNORD116 gene cluster. It has been inserted into the intron region of the pZW1 vector for experimental purposes [PMC4288147]. In a study using HeLa cells, overexpressed sno-lncRNA from pZW1-snoVectors was analyzed for its half-life. The cells were treated with actinomycin D, and the RNA was collected and analyzed using Northern blots [PMC4288147]. In postmortem posterior hypothalamus from individuals with Prader-Willi syndrome, the expression of SNORD116-13 was found to be decreased [PMC5315547]. Additionally, decreased expression of SNORD116-13 was observed in Lymphoblastoid cell lines (LCLs) from individuals with Alzheimer's disease compared to healthy controls. Conversely, LCLs exhibiting higher sensitivity to amyloid-beta (Aβ) showed increased expression of SNORD116-13 [PMC5315547]. The expression levels of several genes, including RGS2, DLGAP1, BCHE, DNASE1L3, SIRT1 and SARM1 were also presented in scatter plots [PMC5315547]. Microarray data for SNORD116-13 were validated using real-time PCR and showed higher expression levels in LCLs with high Aβ sensitivity and lower expression levels in AD LCLs compared to healthy controls [PMC5315547]. The real-time PCR analysis used the SYBR Green technique for both SNORD116-13 and a reference gene GUSB [PMC5315547]. Additionally, sno-lncRNA2 spans from SNORD116-13 to SNORD116-14 according to genomic coordinates provided in another study [PMC7536080].