dre-mir-456: Dre-mir-456 is a miRNA family that has been studied in the early development of chicken and zebrafish [PMC3932949]. In zebrafish, the dre-mir-456 family was found to be highly expressed in the 16-cell stage [PMC3932949]. Quantitative real-time PCR assays were performed to determine the expression levels of dre-mir-456, along with three other known miRNAs (dre-miR-22a, dre-miR-206, and dre-miR-192) [PMC3932949]. These miRNAs were selected based on their known or potential roles in zebrafish early development [PMC3932949]. Additionally, the expression profiles of dre-mir-456 and other non-human miRNAs from zebrafish were quantified to confirm their presence in human cells and body fluids [PMC5478184]. The mean Ct value for dre-mir-456 was found to be 38.77 [PMC5478184]. The use of non-human miRNAs from zebrafish (such as dre-mir-456) that are not ingested in human food was important for confirming a threshold for quantification [PMC5478184]. Overall, these studies highlight the significance of dre-mir-456 and its potential role in early development.