dme-miR-2b: dme-mir-2b is a member of the miR-2 family in Drosophila, and it shares the same mature sequence with dme-mir-2b-1 and dme-mir-2b-2, but is one nucleotide longer than the miRBase annotated sequences [PMC3268580]. The roles and functions of dme-mir-2b in Drosophila are not yet clear [PMC9100521]. Overexpression of dme-mir-2b, along with dme-mir-184 and dme-mir-274, resulted in almost total lethality during embryogenesis/development [PMC9100521]. Overexpression of dme-mir-2b specifically led to a similar outcome [PMC9100521]. Seven miRNAs, including dme-mir-2b, were found to be differentially expressed between long and short photoperiods [PMC9100521]. The overexpression of dme-mir-2b, along with other miRNAs, using UAS transgenes driven by Act-Gal4 or pdf-Gal4 resulted in changes in photoperiodic diapause [PMC9100521]. The microarray experiments indicated that both dme-mir-2b and dme-mir274 are expressed at higher levels in long days compared to short days [PMC9100521]. References: [PMC3268580] - Marco A. Mendoza-Mendoza et al. (2013) Evolutionary Conservation of microRNA Regulatory Circuits: An Examination of microRNA Gene Complexity and Clustering in Mammals, Fishes, Xenopus ,and Caenorhabditis elegans. PLoS ONE 8(6): e66415. [PMC9100521] - Hui Zhang et al. (2020) Identification and Functional Analysis of Differentially Expressed miRNAs in the Photoperiodic Diapause of the Onion Fly, Delia antiqua. Frontiers in Physiology 11: 573.