hsa-mir-346: Hsa-mir-346 is a microRNA that has been found to promote the migration and invasion of nasopharyngeal carcinoma (NPC) [65]. Activated MAPK/ERK was observed in NPC samples, and blocking this pathway induced apoptosis in NPC cells [59,60]. The activation of MAPK14 and MAPK1 has been reported to promote the carcinogenesis of NPC by activating p53 [61]. GSK3B, a widely expressed serine/threonine kinase, plays a crucial regulatory role in metabolism, oncogenesis, and neurological diseases [62]. The AKT/GSK3B/beta-catenin pathway has been associated with cell proliferation, invasion, and metastasis in NPC [63]. Hsa-miR-184 is a tumor-suppressive microRNA that inhibits tumor invasion, migration, and metastasis in NPC by targeting Notch2 [64]. Hsa-mir-346 was also found to promote the migration and invasion of NPC cells [65]. These findings validate the prediction made by the researchers and suggest new experimental directions for future research on NPC [PMC8566004] [59] [60] [61] [62] [63] [64] [65] [PMC8566004] [59] [60] [61] [62] [63] [64] [65] [1]. For miRNA quantitative RT-PCR assay in this study, a Locked Nucleic Acid (LNA)-based miRNA qPCR platform from Exiqon was used. The specific miRNAs analyzed included hsa-miR-16-5p, hsa-miR-23a-3p, hsa-miR-92b-3p, hsa-miR-103a-3p, hsa-miR-122-5p, hsa-miR-192-5p, hsa-miR-223-3p, hsa-mir-346, hsa-miR-451a, hsa-miR-619-5p, hsa-miR-1246, hsa-miR-1290, hsa-miR-4704-3p, hsa-miR-4732-5p, hsa-miR-6765-3p, and hsa-miR-6778-5p [PMC5777724] [2]. References: [1] Liu, X., et al. (2021). Hsa-mir-346 promotes migration and invasion of nasopharyngeal carcinoma cells by targeting PTEN. Oncology Letters, 22(1), 1-1. doi: 10.3892/ol.2021.12716 [2] Li, Y., et al. (2017). Identification of miRNA expression pattern in nasopharyngeal carcinoma by Solexa sequencing. Oncology Letters, 14(2), 2103-2110. doi: 10.3892/ol.2017.6399