dme-mir-975: Dme-mir-975 is a microRNA that was found to be less efficiently cleaved by Dicer compared to dme-pre-let-7 and dme-pre-miR-2492 [PMC8761633]. Overexpression of dme-mir-975 resulted in the down-regulation of six out of the nine predicted targets [PMC8581666]. In contrast, overexpression of dsi-miR-975, despite having a higher expression level than dme-mir-975, did not repress any targets [PMC8581666]. The expression of both dme-mir-975 and dsi-miR-975 was found to be high in S2 cells after transfection, with miR-975 not being detected in S2 cells transfected with the ub-Gal4 control [PMC8581666]. Reporter assays conducted in D. melanogaster S2 cells showed that both the dme-mir-975 and the dsi-mir-975 fragments, along with a ub-Gal4 driver, had trans effects on target repression [PMC8581666]. The expression levels of nine predicted targets with an "A" site complementary to the ninth base of mature dme-mir-975 were examined [PMC8581666]. Interestingly, miRNA knockout experiments revealed that knockout of dsi-mir-975 caused modest but significant target derepression, while knockout of dme-mir-975 did not have a similar effect [PMC8042761].