hsa-mir-520f: Hsa-mir-520f is a down-regulated miRNA that is part of a network of miRNAs, which includes 9 hub miRNAs, including 6 up-regulated miRNAs (hsa-miR-15b, hsa-miR-130a, hsa-miR-142-5p, hsa-miR-1246, hsa-miR-675, and hsa-miR-143) and 3 down-regulated miRNAs (hsa-mir-520f, hsa-miR-588, and hsa-miR-383) [1]. However, the expression trends of hsa-mir-520f were not consistent with the GEO data [2]. Hsa-mir-520f does not have any hub genes that can be used as target genes [2]. In PC3 cells, there are 7 differentially expressed miRNAs, including hsa-mir-433-3p, hsa-mir-154, hsa-mir-324 5p, hsa-miR-509-pre, hsa-mir-377, hsa-mir-520f, and hsa-mir-384 [3]. Additionally, it has been found that hsa-miR-125b-2, hsa-mir-520f, hsa-miR-3175, and hsa-miR-4672 can regulate the expression of target genes associated with immune and angiogenesis processes [4]. In hepatocellular carcinoma (HCC) tissues, there are 5 up-regulated miRNAs (hsa-miR-106b, hsa-miR-25, hsa-miR-93, hsa-miR-222, hsa-miR-221) and 5 down-regulated miRNAs (hsa-miR-424, hsa-mir-520f, hsa-miR-29c, hsa-miR-101, hsa-miR-422a) [5]. In a high-risk group, hsa-mir-520f is overexpressed [6]. References: [1] PMC5041942 [2] PMC8612537 [3] PMC6874298 [4] PMC8779150 [5] PMC6171011 [6] PMC5040965